AlleleID by PREMIER Biosoft is a highly specialized desktop software application designed for complex real-time PCR (qPCR) assay design. It is primarily utilized for bacterial identification, pathogen detection, species discrimination, and allele identification.
Unlike general tools like Primer3, AlleleID incorporates structural analysis and alignment directly into the pipeline. Below is a structured guide on how to navigate the software for real-time PCR primer design. Key Real-Time PCR Chemistries Supported
Before starting, choose the detection chemistry needed for your experiment: SYBR Green: Intercalating dye-based detection.
Fluorogenic Probes: Designs compatible pairs alongside TaqMan, TaqMan MGB, Molecular Beacons, and FRET probes. Step-by-Step Design Workflow 1. Setup Your Project and Input Sequences Real-Time PCR Primer Design – PREMIER Biosoft